Endotoxin translocation in two models of experimental acute pancreatitis

Role of S-adenosylmethionine in two experimental models of pancreatitis.

Severe necrotizing pancreatitis occurs in young female mice fed a choline-deficient and ethionine-supplemented (CDE) diet. Although the mechanism of the pancreatitis is unknown, one consequence of this diet is depletion of hepatic S-adenosylmethionine (SAM). SAM formation is catalyzed by methionine adenosyltransferases (MATs), which are encoded by liver-specific (MAT1A) and non-liver-specific (...

Experimental Models of Pancreatitis

Acute pancreatitis is an inflammatory disease characterized by interstitial edema, inflammatory cell infiltration, and acinar cell necrosis, depending on its severity. Regardless of the extent of tissue injury, acute pancreatitis is a completely reversible process with evident normal tissue architecture after recovery. Its pathogenic mechanism has been known to be closely related to intracellul...

Experimental Models in Syrian Golden Hamster Replicate Human Acute Pancreatitis

The hamster has been shown to share a variety of metabolic similarities with humans. To replicate human acute pancreatitis with hamsters, we comparatively studied the efficacy of common methods, such as the peritoneal injections of caerulein, L-arginine, the retrograde infusion of sodium taurocholate, and another novel model with concomitant administration of ethanol and fatty acid. The severit...

Clinical relevance of experimental models of acute pancreatitis

flow cytometric analysis of inflammatory cells in experimental acute pancreatitis

background: accumulating evidence indicates that inflammatory cells migrate into the pancreas tissue and play an important role in the pathogenesis of acute pancreatitis (ap). the aim of this study was to establish a flow cytometric method to enumerate these infiltrating cells in the pancreas of an experimental ap. materials and methods: twelve hours after inducing of ap, mice pancreatic tissue...